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While the zinc oxide group showed significantly greater wound contraction than the control group on days 14 and 21, its performance was not as rapid as TCC in the initial phase

The lysates were centrifuged (10.000 g , 30 min, 4 C) and the supernatants were loaded on equilibrated Ni-NTA agarose columns (Qiagen)

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In contrast, a GSSR intermediate was detected and its decreased steady-state concentration at higher glutaredoxin concentrations was suggested to support monothiol mechanism (i) 60