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GLP-1 receptors are expressed in brain regions involved in appetite and energy regulation, including the hypothalamus and brainstem

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After incubating for another 12 h at 160 rpm at 16 C, the cells were collected, and homogenized in a GST binding buffer (PBS supplemented with 10 mM -mercaptoethanol and 1% Triton X-100) on ice

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This ramp-up phase typically takes 4-12 weeks depending on the specific medication and your response