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Mechanistically, TMAO may contribute to kidney disease pathogenesis by inhibiting autophagy, activating the NLRP3 inflammasome, and inducing mitochondrial dysfunction

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- Cells not handled gently: Freezing and thawing procedures are stressful to most cells

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The receptors are expressed in pancreatic -cells, mature adipocytes, certain hypothalamic neurons, hindbrain nuclei, and a few other tissues directly involved in metabolism