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If the GST moiety is to be cleaved away from the protein of interest, the pGEX-T series contains a protease cleavage site for thrombin, the pGEX-X series of vectors contain protease cleavage sites for factor Xa, and the pGEX-P series contains a cleavage site for PreScission protease (see Figure 2)

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Thus we have targeted the dimerization process by developing molecules that mimic the dimerization domain, the hinge region, with idea that they can act as dominant negative replacements