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This result was confirmed statistically using a non-parametric analysis of variance (KruskallWallis test), followed by post-hoc analysis (Wilcoxon method) that showed the procedural blank froze at a significantly different temperature than all of the compounds (Supplementary Table 2)

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This double effect of decreased production and increased consumption makes boosting NAD+ levels a potential strategy for supporting cellular health as we age 1

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Wash steps prior to proteolysis were carried out with 200 L of 20 mM ammonium bicarbonate (instead of 100 L)