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After pooling by equal volume
and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter
a shearing homogenizer
parainfluenza virus
This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt)
dsDNA Shearase Plus Size:50 U After pooling by equal volumedsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5 phosphate and 3 hydroxyl termini. It has a particularly strong preference for dsDNA and generates random ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.
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