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75 ug of proteins were reduced in 50 L of 10 mM HEPESKOH pH 7.5, 8 M urea containing Dithiothreitol (DTT) to a final concentration of 5 mM followed by heating at 95 C for 2 min, and a 30-min incubation at room temperature

doi: 10.1007/s12011-019-01995-x 124 AkinciMKosovaFCetinBSepiciAAltanNAslanSet al

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