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coli DH5 strain was transformed with the expression vector pGEX 4T-1 with individual commercially synthesized (Genscript USA Inc.) genes of Cr FAP65AH1, Cr FAP65AH1V12P, Cr FAP65AH2 and Cr FAP65AH2V12P giving rise to Glutathione S-transferase (GST) tagged fusion polypeptides (for details see plasmid construct in Additional file 1: Fig

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