Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole
For best results, start with 1/4 dose and work up to the full dose over the course of 4 to 10 days
[DOI] [PMC free article] [PubMed] [Google Scholar] Cruz P, Torres C, Ramrez ME, Epun MJ, Valladares LE, Sierralta WD, 2010
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It also influences the way the body responds to insulin and supports cognition and mood
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