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These metabolic shifts, combined with reduced caloric intake, can lead to a decrease in thermogenesisthe body's heat production

These byproducts also activate intestinal L cells, boosting the secretion of endogenous GLP-1 and creating a reciprocal feedback mechanism between the host and its microbes [24,25]

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Tail vein blood samples were collected in 10 l heparinized capillary tubes at times 60, 0, 15, 30, 60, 120 and 240 min., and immediately suspended in glucose/lactate solution buffer (0.5 mL, EKF-diagnostics, Cardiff, UK)

However, asynchrony between oocytes and spermatozoa, especially when aged oocytes are fertilized, will affect the rates of fertilization and embryo development, thus reliable methods are needed to control oocyte aging to benefit modern ART [28]

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