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A scientific rational

Chang, D

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A microwave power of 6.3 mW and a modulation amplitude of 5 G at a modulation frequency of 100 kHz were used to acquire spectra in the range of 3367 G to 3497 G at a sweep time of 156.22 s and a conversion time of 78.11 ms

Subsequently, the blots proceeded through a second round of immunodetection by agitating for 45 min at 50C in stripping buffer (2% SDS, 0.8% -mercaptoethanol (ME), and 62.5 mM Tris-HCl, pH 6.8) 32,56,62 followed by re-probing with HRP-labeled anti-HGF N -terminal domain mAb (1:2500 dilution in CanGetSignal solution 1) to monitor HGF -chain amount on the blots as loading controls