For the purpose of preserving muscle during a deficit, what matters is that the last two or three repetitions of each set are genuinely hard and that the load can be progressed over time
Behavioral measurements should primarily include the percentage of time spent in the open quadrant, the percentage of entries into the open quadrant, the number of times the animal peeks down from the edge of the platform while in the open or closed quadrant, and the number of stretch-attend postures from the closed quadrant to the open quadrant
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However, it also has been found to be associated with insulin resistance syndrome including obesity, fat distribution, hypertension, dyslipidemia, and cardiovascular disease [48]
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