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In brief, for the HITI donor vector containing the sgAlb-E14 expression cassette and scExe4 donor fragment, the sgAlb-E14 expression cassette and scExe4 (NGF-FCS2-Exe4) sequence flanked by two sgAlb-E14 gRNA target sequences were prepared by PCR using PrimeSTAR GXL polymerase (Takara Bio)

Acknowledgements Work in the authors' laboratories is mainly supported by the European Research Council (to M.D.P.), the Swiss National Science Foundation (grants 31003A-165963 to M.D.P

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