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Gather Your Supplies: You'll need your vial of IGF-1 LR3, a vial of Bacteriostatic Water, a new sterile syringe (3mL is a good size for this), and alcohol swabs

Concurrently, NCOA4 mediates the autophagy of ferritin, releasing stored iron and further increasing free iron levels within cells (Bellelli et al., 2016)

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hPD-L1 MC38 cells were cultivated in Dulbeccos Modified Eagle Medium containing 10% (v/v) fetal bovine serum (FBS), 50 g/mL hygromycin B, and antibiotics (100 U/mL penicillin and 100 g/mL streptomycin)