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All amino acids were double coupled for 4 min at 75 C, with the instrument set to deliver the N--Fmoc-amino acid solutions (0.2 M solution in DMF), HOBt (1.0 M solution in DMF) and DIC (1.0 M solution in DMF)

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The distinct features manifested by R310 6.40b and H363 6.52b are likely to be responsible for the kink formation induced by compound 2, in line with previous mutagenesis results that mutation at these two sites altered GLP-1R signaling profiles (cAMP, pERK and iCa 2+ ) 4,37

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