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Ligand-induced bioluminescence resonance energy transfer (BRET) assays Twenty-four hours following transfection, HEK293T cells or MIN6 cells were washed with PBS and resuspended in FluoroBrite phenol red-free complete media (catalogue no

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Subcutaneous formalin-induced activity of dorsal horn neurones in the rat: differential response to an intrathecal opiate administered pre or post formalin