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Followed by SDS-PAGE electrophoresis, the transfer of proteins was conducted onto PVDF (Millipore) membranes (Solebio, Beijing, China) and immunocontainment experiments were performed after the membrane transfer was completed, the primary antibody was added and incubated at 4 C overnight with slow shaking
Jurek B, Neumann ID
doi: 10.3390/ijms26167772 218 WdowiakAFilipM
Mast cells participate in the direct defense against invading pathogens in a few ways:10,11,12 Phagocytosis: Mast cells are able to engulf or ingest pathogens to directly neutralize them