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Interestingly, in addition to raised glucose serum levels in alloxan treated animals, an exaggerated appearance of gastric lesions was noted in control diabetic rats

Subsequently, the mice were anesthetized, and blood samples were obtained from the intraorbital venous plexus

10.1210/en.2007-1190 Endocrinology 38 GesingA.BartkeA.WangF.Karbownik-LewinskaM.MasternakM

Subsequently, 100 L of biotinylated detection antibody working solution was added to each well, and incubated for 1 h in the dark

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The main reason for this was a high drop out rate of 6 out of 13 subjects enrolled because of AEs