Myth: If I am still hungry, the medication isnt working for me
Glob Adv Integr Med Health
Available at: (Accessed October 26, 2020)
The cells were washed with PBS() and sonicated in lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% TritonX-100, 2.5 mM sodium pyrophosphate, 1 mM -glycerophosphate, 1 mM sodium orthovanadate, 10 g/mL leupeptin, 1 g/mL aprotinin, 1 mM phenylmethylsulfonyl fluoride)
40 l of supernatant was removed from each treated well and transferred into a 96-well clear bottom black microplate (Costar, Corning, USA)
This timing issue makes disclosure of all medications to your healthcare provider critical when starting antibiotic treatment