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Description

Immunocytochemistry and imaging Hypothalamic progenitors were re-plated onto PhenoPlate TM 96-well microplates (Perkin Elmer) and hypothalamic cultures at 33-40 days post-differentiation were fixed in 4% paraformaldehyde (Thermo Fisher Scientific) for 10 minutes, rinsed three times with Tris-buffered saline containing 0.1% Triton X-100 (TBS-T

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Results A total of 20 studies, encompassing 44 independent experiments and involving 1019 animals, were included in the analysis

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