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Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

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First, an insulin-secreting -cell line MIN-6 cells stably expressing GLP-1R (GLP-1R-MIN-6 cells) was prepared, as we intended to observe a stronger effect of GLP-1R activation than that of endogenous GLP-1R

Stimple SD, Kalyoncu S, Desai AA, Mogensen JE, Spang LT, Asgreen DJ, et al

Absorption issues often resolve with better adherence to the spacing protocol before any dose change becomes necessary