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The 3.0 mL dilution is deliberately large so low microgram doses read on clear U-100 markings

Similar to this, the transmembrane adhesion molecule glycoprotein basal cell adhesion molecule (Lutheran blood group) and its particular integrin-4-1 mediate the attachment of sickle cells to the endothelium [40]

Thus, since HK2 translation is known to be regulated by an IRES mechanism [34], we performed a luciferase assay to assess whether SYNCRIP affects HK2 expression through IRES-mediated translation

The method used was that described by (14)