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The DNA from both reactions is then amplified using Real-Time PCR
plasmid shuffling
the Quick-DNA/RNA™ Water Kit ensures robust recovery and purification of DNA/RNA
ear punches
RNA Max Buffer is used in RNA recovery and purification from PAGE gels in the ZR small-RNA PAGE Recovery kit
CpG Methylase (M. Sssl) Microbial DNA Isolation The DNA from both reactionsCpG Methylase completely methylates all cytosines in double stranded, non methylated, and hemimethylated DNA in a 5' CpG 3' context. The recombinant methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions have been optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis.
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