2016 Nov 29;13(4):e12394
S2.4 Comparison between HH curves, pHp, and amount of niclosamide in solution To complete this theoretical assessment, plotted in Figure S5 (red symbols) is the pHp curve, giving the maximum amount of niclosamide expected to be in solution (uM) versus the solution pH (right axis) using the values derived from experiment of pKa = 6.87, S o = 1.77uM
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In other words, the stretch causes the second layer to tear, and the third layer, beneath the torn tissue, to become visible
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Step 9: Inject the Solution Slowly and steadily push the plunger