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Quantification of lipogenesis gene expression To quantify gene expression, we used the Pure Link RNA Mini kit (12183025, Invitrogen, USA), Hilden, Germany) to extract total RNA from untreated and treated HepG2 cells and used High-Capacity cDNA Reverse Transcription kit (4368813, Applied Biosystems, Foster City, CA, USA) and 2 g total RNA to prepare cDNA

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PMID: 87 KlyneDMBarbeMFJamesGHodgesPW

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