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### Step 7: Inspect the Solution The reconstituted solution should be: - Clear (not cloudy or hazy) - Colorless (no yellow or brown tint) - Free of visible particles or floaters - Completely dissolved -- no powder clinging to the vial walls **If any of these criteria are not met, do not inject.** Cloudiness or particles indicate degradation or contamination

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For instance, in mouse embryonic stem cells, targeted disruption of glucose-6-phosphate dehydrogenase, which is the rate-limiting enzyme in the pentose phosphate pathway, the predominant metabolic pathway for the generation of NADPH (a cofactor of GR), renders cells extremely sensitive to H 2 O 2 -induced cell death 34

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