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Rabbit anti-GLP-1R antibody, mouse anti-glial fibrillary acidic protein (GFAP) and mouse anti-alpha smooth muscle actin ( -SMA) were from Abcam PLC (Cambridge, UK), Goat anti-type I collagen antibody from Southern Biotechnology (Birmingham, AL, USA), rabbit antibody against glyceraldehydes-3-phosphate dehydrogenase (GAPDH) from Trevigen (Gaithersburg, MD, USA) and anti-rabbit IgG Alexa488-conjugated antibody, anti-mouse-IgG-Alexa555-conjugated antibody, anti-goat-IgG-Alexa350-conjugated antibody and Hoechst33342 were from Invitrogen (Carlsbad, CA, USA)
Baseline kidney function testing and monitoring at 612 month intervals provides reassurance and allows dose adjustments if renal function declines from other causes
Additionally, non-enzymatic binding of glucose to circulating proteins results in generation of AGEs
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