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Western blot analysis Total proteins were extracted from human trophoblast Swan 71 cells, human villous tissues, and mouse placental tissues using RIPA lysis buffer (Thermo Fisher Scientific) and quantified using Pierce BCA Protein Assay Kit (Pierce)

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During this time, the demands of the brain increase and BDNF (Brain-Derived Neurotrophic Factor) is also released (1, 2)

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