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After TCA precipitation, the pellet was washed with ice-cold acetone (200 L) twice, dried, and dissolved in 49.5 L of DNase buffer (40 mM TrisHCl pH 7.5, 8 mM MgCl 2 , and 5 mM DTT)

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Some patients may reduce frequency or dosing once optimal levels are achieved and maintained

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Even though this phenomenon has not been fully understood in the context of the possible exacerbation of pro-depressive changes by GLP-1RA administration, it certainly requires careful investigation before the potential introduction of these compounds to the clinic for the treatment of mood disorders in the future