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We hypothesized that if the interaction site is comparable between the WD40 and WD40 domains, then a disruptive substitution at a juxtaposed critical WD40 residue should yield a similar weakening of S tail interaction with either domain

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Yet, de novo choline synthesis in humans is not sufficient to meet their metabolic needs such that healthy humans fed choline-deficient diets develop fatty liver, liver damage, and/or muscle damage (see Deficiency)

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