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Dissociated hippocampal neurons were transfected with mRFP-p-actin and shMet RNA and receptor knock-down was allowed to take place for 48 hours prior to stimulating with 0.5 g (per well) HGF (10 ng/ml), Dihexa or Nlel -AnglV (both at 10 " 12 M)

Allow it to stay for a few minutes, and then wash it off

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Indeed, we found hypotaurine and serine levels increased in the liver of Gclc KO mice (Fig