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The exact wait time can vary, so be sure to follow your provider's advice

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For protein extraction, each tissue was placed into a tube containing a lysis buffer consisting of 20 mM Tris HCl with pH 7.6, 0.5 M Sucrose, 0.15 M KCl, 20 mM DTT supplemented with 100 L/mL of a protease inhibitor cocktail (Sigma-Aldrich, MO, USA) in a 1:3 (v/v) ratio

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Cytokine (2000) 12(7):113641.10.1006/cyto.1999.0643 23 GuNTsudaMMatsunagaTAdachiTYasudaKIshiharaAet alGlucose regulation of dipeptidyl peptidase IV gene expression is mediated by hepatocyte nuclear factor-1alpha in epithelial intestinal cells