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The labelling was done using anti-PDGFR antibodies (R&D Systems, AF1042, 1:50) for pericytes, isolectin GS-IB4-biotinylated (Life Technologies, 121414, 1:200) for capillary basement membrane, and anti-Kir6.1 (Alomone Labs, APC-105, 1:100) to localise the K ATP channels, following heat-induced epitope retrieval (using 10 mM sodium citrate buffer at pH 6.0 for 20 min at 80 C)

Core operating margin : Increased to 35%, up by three percent

Hansen SN, Tveden-Nyborg P, Lykkesfeldt J