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Western blotting Whole lysates, mitochondrial or nuclear fraction were extracted with either lysis buffer containing a protease/phosphatase inhibitor cocktail or other appropriate kits as previously reported 6,7
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One of the prevailing theories suggest that increased hepatic FFA flux can impede the efficiency of the mitochondrial transport chain during energy production, and in the process, cause the excessive production of reactive oxygen species (ROS)
An improvement of oxidative stress in diabetic rats by ubiquinone-10 and ubiquinol-10 and bioavailability after short- and long-term coenzyme Q10 supplementation
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