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Specifically, after plating each cell line at a concentration of 5 10 4 cells per well, the cells were incubated with NAPQI for 24 h at seven concentrations ranging from 0 to 100M dissolved in 1% DMSO

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Conclusion The current study enumerates a step-by-step design of experiments for the solubilization of IB proteins (probably of the non-classical type) for two amphipathic helices harboured on an AKAP (FAP65) that were surprisingly insoluble even after fusion with GST, a commonly used solubility-enhancer tag while producing recombinant proteins