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Following purification, the protein was diluted to 0.08 mg/mL in refolding buffer at pH 8.5 (10.56 mM NaCl, 55 mM Tris-HCl, 2.2 mM CaCl2, 2.2 mM MgCl2, 0.055% PEG4000, 0.55 M L-arginine, 0.1 mM oxidized glutathione, and 1 mM reduced glutathione) and incubated overnight at 4C with shaking
Wakabayashi N, Skoko JJ, Chartoumpekis DV, Kimura S, Slocum SL, Noda K, et al
Nitta K, Tsuge H, Sugai S, Shimazaki K: The calcium-binding property of equine lysozyme
Together, these four peptides form what is referred to as the KLOW Blend, because their actions are hypothesized to be partly overlapping and partly complementary based on the available research data
Its about ensuring that when your feet hit the floor in the morning, your joints feel limber and your body feels revitalized