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Liver tissue and EVs from mice at different stages of PCOS, revealing complex metabolic interactions between liver tissue and EVs, where the downregulation of glycolysis and the tricarboxylic acid cycle may be related to the hepatic pathophysiology of PCOS, independent of age ( 3.1.4 EVs regulate sugar and lipid metabolism and affect obesity Obesity promotes the development of PCOS, which in turn exacerbates obesity (Teede et al., 2023)