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The method for synthesizing Cagrilintide according to claim 1, wherein in step three, 200mL of cleavage reagent is prepared, the fully protected peptide is added under ice bath conditions, the reaction is continued for 2h after 0.5h of return to room temperature, after the reaction is finished, anhydrous ethyl ether is added for precipitation, the centrifugal precipitation is carried out for multiple times, 300mL of ethyl ether is added each time, and the product obtained after drying is Cagrilintide peptide

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The protein bands were detected using ECL detection reagents (Thermo Scientific) and exposed on blue X-ray film (Phenix Research Products, Candler, NC)

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