Following an overnight incubation at 4C in blocking buffer containing Gpa-GSS17 antibody at a dilution of 1 in 200, nematodes were washed, reblocked for 30 mins and incubated with FITC-conjugated goat anti-rabbit 2 o antibody (Sigma) at a dilution of 1 in 200 for 2h at room temperature
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BPC-157, or Body Protection Compound-157, is a synthetic peptide derived from a protective protein found in the human stomach
After centrifugation at 5000 rpm for 10 min, cells were incubated with primary antibodies FSP1 (1:500, Proteintech, 20886-1-AP) and importin 2 (1:200, Santa Cruz, SC-55538) at 4 C overnight