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Serial diluted GIPR-Ab was incubated with 30,000 cells in assay buffer (0.1% BSA, 500 mM 3-isobutyl-1-methylxanthine in F12 media) for 30 min at 37 C before treatment with GIP at a final concentration of 0.05 nM

Plant Physiology 133, 748760

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Both hormones are released from intestinal enteroendocrine cells in response to nutrient intake, but their mechanisms of action differ in important ways

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The semaglutide group, initially losing an average of 17.3% of their body weight, returned to 5.6% below their starting weight