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RTqPCR Total RNA was isolated using the RNeasy Mini Kit (Qiagen, 74104) and reverse-transcribed with a high-capacity RNA-to-cDNA kit (Vazyme, R323-01)

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10.1016/j.molmet.2015.07.008 [DOI] [PMC free article] [PubMed] [Google Scholar] Coskun, T