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Glucagon and insulin cooperatively stimulate fibroblast growth factor 21 gene transcription by increasing the expression of activating transcription factor 4

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The protein was finally eluted with buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 0.01% (w/v) MNG, 0.001% (w/v) CHS, 200 mg/ml Flag peptide, 5 mM EDTA and 1 M ghrelin or ibutamoren, and further purified by size-exclusion chromatography on a Superdex 200 Increase column (Cytiva) using buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 0.01% (w/v) MNG, 0.001% (w/v) CHS, and 1 M ghrelin or ibutamoren

The authors concluded that tobacco seeds were particularly sensitive to accurate SAM/SAH levels at critical developmental periods

Tang, X., Liu, J., Dong, W., Li, P., Li, L., Lin, C., Zheng, Y., Hou, J., Li, D