These data are strongly supported by clinical studies that showed decreased miR-200b in serum and cerebrospinal fluid of AD patients, compared to healthy subjects (Silvestro et al., 2019)
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No significant alterations to mitochondrial mass were demonstrated following metformin treatment in HCT116 cells, however, in SW837 cells, mitochondrial mass was significantly increased following 24 h treatment with metformin (10 mM), when compared to control ( Figure 1C )
This increase at low oxidative stress is due to the stimulation of CBS and GCS that increases GSH synthesis and concentration, and therefore the rate of export
Notably, genes involved in the TSS pathway, cystine uptake and glutathione recycling (such as CBS, CSE, SLC7A11 and GSR) were DNA hypomethylated after DMF treatment 30 , supporting a regulatory effect of DMF on these pathways