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The collected cells were suspended in 300 L ice-cold TE buffer (100 mM TrisHCl (pH 8.0), 2 mM ethylenediaminetetraacetic acid) and disrupted by a cell disruptor (Shake Master NEO, Bio Medical Sciences, Tokyo, Japan) with zirconia beads at 1500 rpm for 10 min

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These microorganisms produce ACC deaminase, which reduces the effects of salt stress by lowering the levels of the plant hormone ethylene

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