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For example, the tripeptide glutathione is synthesized in two steps from free amino acids, by two enzymes: glutamatecysteine ligase (forms an isopeptide bond, which is not a peptide bond) and glutathione synthetase (forms a peptide bond)

DUODENOCUTANEOUS FISTULA IN RATS AS A MODEL FOR WOUND HEALING-THERAPY IN ULCER HEALING: THE EFFECT OF PENTADECAPEPTIDE BPC 157, L-NITRO-ARGININE METHYL ESTER AND L-ARGININE INTRODUCTION We wanted to challenge the understanding of duodenal ulcer disease pathophysiology and therapy, along with Selyes and Szabos cysteamine duodenal lesions, as the mainstay in the duodenal ulcer disease model (1-3), which argues against wound healing therapy (i.e., duodenal ulcer heals like the skin ulcer does)

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Neuropharmacology 67:337348