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In contrast, the other two substitution mutants, GSAAT(C168S) and GSAAT(C190S), and the corresponding double mutant, displayed hardly any increase in activity under reducing conditions
2.6 Quantitative PCR (qPCR) analysis To quantify the transcript levels of various inflammatory cytokines (TNF-, IFN-, IL-1, IL-18, IL-6, and IL-23), colonic mRNA expression was analyzed through quantitative PCR
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In the Type I syndrome patients have hypoparathyroidism, muco-cutaneous candidiasis, Addison's disease, and occasionally hypothyroidism
Discussion In the last decade many efforts were employed in research and development of new pharmacological treatments of FXS and simultaneously great advances were made in developing peptides therapeutics against several diseases
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