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Off-label prescribing accounts for roughly 20% of all prescriptions written in the United States

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it freely crosses the cell membrane and inhibits UCP2-mediated proton leakage in mitochondria, increases the mitochondrial membrane potential, increases ATP levels, and closes K (ATP) channels without affecting other mitochondrial functions (Zhang et al., 2006)

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ROS levels were quantified by observing cells with an inverted fluorescence microscope and measuring the average fluorescence intensity within Myocytes using Image J software after pre-culturing with various concentrations of AX for 6 hours, followed by 2 hours of thermal stress at 37C, with 6 replicates per group

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