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hPSC lines were seeded into 12 well plates 1 day prior to initiation of the EP screen

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This 2019 research was intended to be a pilot study, and the researchers acknowledged that, "More small dogs from different breeds should be enrolled in future studies." They also disclosed that: "Second, five [of the ten] MMVD dogs

Brief exposure for example, during a single research session typically doesnt meaningfully affect the solution, but extended time outside refrigeration accelerates degradation and shortens the usable window described above