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Reactive oxygen species and mitochondria: A nexus of cellular homeostasis

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Key GPX1 Variants rs1050450 (Pro198Leu) Leu allele carriers: Reduced GPX1 activity, increased oxidative stress

[DOI] [PMC free article] [PubMed] [Google Scholar] 135.Fatemi SH, Aldinger KA, Ashwood P, Bauman ML, Blaha CD, Blatt GJ, et al

Analysis of mRNA expression using quantitative polymerase chain reaction Total RNA was extracted from cells or tissue using the TRIzol reagent (Life Technologies) following the manufacturer's instructions

Protocols typically involve 10 to 40% reductions in total energy intake compared to ad libitum-fed controls, but with adequate nutrition and a controlled physical environment (2)