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[DOI] [PMC free article] [PubMed] [Google Scholar] 30.Javed Z., Papageorgiou M., Deshmukh H., Rigby A.S., Qamar U., Abbas J., Khan A.Y., Kilpatrick E.S., Atkin S.L., Sathyapalan T

Key factors include antibody quality and specificity, choice of detection substrate, assay format (sandwich, direct, indirect), sample preparation, and environmental conditions like temperature and incubation times

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They produce the alpha-melanocyte stimulating hormone (alpha-MSH) that binds to the melanocortin 3 and 4 receptors (MC3Rs and MC4Rs) expressed by the second order neurons located in the periventricular nucleus and the lateral hypothalamic area (LHA) (51) (Figure 1)